Facsaria software

Please fill in the following information and we will get in touch with you regarding your query. The fluidics and optical systems are precisely integrated to maximize signal detection. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve high sensitivity and resolution.

Differentiating H9 cells. This combination of markers has been widely used to characterize and isolate differentiated and undifferentiated stem cells derived from hESCs and iPS cells. Representative FoxP3 staining of sorted Tregs. Data is representative of 10 experiments. This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.

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Do you want to continue? The flow cell and nozzle design enable low particle speeds in the analysis zone for maximum light collection, and then accelerate the particle through the nozzle at stream speeds to achieve the drop rates required for high-performance sorting.

Through the precise coordination of the optical and fluidics systems, the BD FACSAria III delivers exceptional optical detection sensitivity compared to traditional stream-in-air systems, in which particle speeds are the same for both analysis and sorting. Nozzles for a range of particles A choice of nozzles lets users sort a wide range of particle sizes.

Nozzles are available in four sizes: 70, 85, , and microns. Nozzles are readily accessible and easy to change, with a design offering tight registration for a secure fit. This means a reproducible drop profile after every nozzle exchange, resulting in reproducible instrument setup and alignment. The software sort setup matches pressure and sort settings to the nozzle being used. Easy aseptic setup and cleaning Innovations in the fluidics system such as easy-to-insert nozzles, automated sort setup, and easy-to-change filters make setup fast and simple.

The fluidics design features integrated valve manifolds and a streamlined fluidics path. Software wizards make aseptic sort setup easy and effective. In addition, after a sample tube is run, both the inside and outside of the sample injection tubing are flushed to minimize carryover. Software automation simplifies drop-delay determination.

Once the drop-delay is calculated, the system automatically adjusts to maintain a constant break-off, called the Sweet Spot. Automatic clog detection stops the sort and protects the collection tubes if a clog is detected.

After passing through the cuvette, the stream accelerates through the nozzle, and droplets are formed for sorting. Since particle interrogation occurs above the nozzle, insertion and removal of the nozzle can occur without realigning the optics or the fluid stream. Engineered into the System A self-contained fluidics cart supplies sheath and cleaning fluids and collects waste from the cytometer. The cart also provides the air pressure and vacuum needed to achieve pressure from 5 to 75 psi, to accommodate a variety of cell sorting applications.

The fluidics cart is typically positioned directly under or to the left of the cytometer. The fluidics cart holds a L stainless steel sheath tank, a 5-L stainless steel tank used for shutting down the instrument with ethanol, and L waste container.

The sheath tank can be autoclaved. In addition, the cart holds three 5-L auxiliary cleaning fluid containers used in conjunction with the automated Prepare for Aseptic Sort mode. Sample injection chamber During acquisition, the sample injection chamber is pressurized, forcing the sample to the cuvette flow cell. A variety of tube holders are provided, from mL centrifuge tube to 1. To minimize clogging, and micron sample line filters are available.

From the sort block to the collection chamber After leaving the nozzle, particles pass through the sort block that houses the deflection plates. The novel design fixes the plates in position for more efficient and reproducible deflection into a collection device in the sort collection chamber.

The sort block also houses an aspirator drawer that keeps the sort collection tubes covered until sorting begins and automatically closes to protect the tubes when the Sweet Spot is on and a clog is detected. The holders are designed to help maintain aseptic conditions when removing sort tubes.

Temperature control for sort collection tubes, slides, and plates is available as an option. Increased efficiency for multicolor detection Innovations in the optical system, pioneered by BD, efficiently maximize signal detection and greatly increase sensitivity and resolution for each color in a multicolor assay.

Enhanced sensitivity and resolution mean that even dim populations can be readily identified and sorted. The optics system allows optimizing multicolor assays and panel design for superior results. The design provides researchers a choice of laser excitation wavelength s that illuminate cells in the sample.

Collection optics direct light scatter and fluorescence signals through spectral filters to detectors. Innovative designs for both the excitation and collection optics reduce excitation losses and dramatically improve collection efficiency, yielding better information from each sample. The more concentrated the beam spot, the higher the signal produced as each fluorescent-labeled particle passes through the laser spot. Laser light is focused into the gel-coupled cuvette flow cell.

Optical gel coupling to the fluorescence objective lens enables transmission of the greatest amount of emitted light from the interrogation point to the collection optics.

Since the optical pathway and the sample core stream are fixed, alignment is constant from day to day and from experiment to experiment. Fixed alignment also ensures that there is no variability in experiment results introduced by manual optical adjustments.

Collection optics Fiber optics deliver emitted light from the gel-coupled cuvette to the detector arrays. The collection optics are set up in patented octagon pathways that maximize signal detection from each laser illuminated beam spot.

This is accomplished by transmitting the highest wavelengths which have the fewest photons of light to the first photomultiplier tube PMT , and reflecting lower wavelengths to the next PMT through a series of longpass dichroic mirrors. This design is based on the principle that light reflection is more efficient than light transmission. Emitted light travels to each PMT via reflection and is transmitted through only two pieces of glass to reach each detector. Therefore, colors can be detected with minimum light loss.

Bandpass filters in front of each PMT allow spectral selection of the collected wavelengths. Importantly, this arrangement simplifies filter and mirror changes within the optical array and requires no further alignment, for maximum signal strength.

This precision design delivers a more efficient optical system enabling the use of lower powered lasers, which in turn reduces the total cost of instrument operation. Flexibility to Expand, Upgrade to Protect Investments For many users, the capability of an advanced cell sorter is defined by its flexibility, which in turn is defined by the number of parameters that can be detected simultaneously.

Choose up to six laser wavelengths— nm, nm, nm, nm, nm, and nm—and up to 20 detector positions, to measure up to 18 colors simultaneously. Lower cost of ownership The unique, efficient design of the optical system delivers a lower cost of operation than stream-in-air sorters. No special power or cooling is needed for these lower powered air cooled lasers. Researchers gain application flexibility because it is easier to move the assay design and optimization to another platform, for example, from analysis to sorting.

The software reduces the chances of operator error, and ensures consistency of results. It allows for the creation of application-specific settings for rapid performance of routine experiments in a more consistent manner.

Tracking capabilities in the software measure a number of instrument settings and report on performance, simplifying daily quality control. Levey-Jennings plots help users understand instrument performance and identify maintenance issues. Acquisition and analysis BD FACSDiva software enables researchers to preview and record data from multiple samples with an automated acquisition process. The software manages acquisition templates, experiment layouts, and compensation procedures to further facilitate data acquisition.